1.
Article
in English
| IMSEAR
| ID: sea-16630
ABSTRACT
Four anti-alphafoetoprotein (AFP) monoclonal antibodies (MAb) were raised in the laboratory and characterized using ELISA and immunodot assays. The affinity constants of the MAbs, analysed by scatchard plots, ranged from 3.1 X 10(8) to 2.15 X 10(9) M/l. Epitope analysis using competition RIA indicated that MAb 5E2D7 and 5E2E3 recognize different epitopes on AFP. This combination was used to set up a two site sandwich ELISA with HRPO conjugated 5E2D7. AFP values in sera of hepatocellular carcinoma patients and pregnant women were quantitated using sandwich ELISA. The anti-AFP MAbs showed strong reactivity when tested on hepatoma tissue sections using immunoperoxidase technique.
Subject(s)
Antibodies, Monoclonal/analysis , Carcinoma, Hepatocellular/analysis , Enzyme-Linked Immunosorbent Assay , Female , Humans , Immunoblotting , Liver Neoplasms/analysis , Pregnancy , alpha-Fetoproteins/immunology
2.
Indian J Pathol Microbiol
;
1980 Jul; 23(3): 189-91B
Article
in English
| IMSEAR
| ID: sea-75762
3.
Indian J Cancer
;
1974 Sep; 11(3): 296-300
Article
in English
| IMSEAR
| ID: sea-49413
Subject(s)
Acetamides , Animals , Carcinoma, Hepatocellular/analysis , Liver/enzymology , Liver Glycogen/analysis , Liver Neoplasms/analysis , Male , Mice , Mice, Inbred C3H , Thioacetamide
4.
Indian J Biochem Biophys
;
1974 Jun; 11(2): 176-8
Article
in English
| IMSEAR
| ID: sea-26408